PyroDoQ - FAQ

1. What is PyroDoQ?
2. What is the basic idea behind the simulation used by PyroDoQ?
3. What is the advantage of simulation?
4. Can I use PyroDoQ for DQ & validation of chromogenic and turbidimetric LAL-kinetic measurement techniques?
5. Is Signal Processing used in PyroDoQ & What is Signal Processing?
6. Can I simulate curve smoothing in PyroDoQ?
7. Can I simulate "noise" conditions in PyroDoQ?
8. Can I generate outlier(s) in PyroDoQ?
9. Can I simulate Robust Kinetic measurement?
10. Can I use PyroDoQ for other applications?
11. Can I integrate PyroDoQ with other Opulus applications?

1. What is PyroDoQ?
   PyroDoQ is the first and only product, which provides Design Qualification (DQ) for LAL-endotoxin kinetic test system. In addition to DQ compliance, PyroDoQ can generate various scenarios for evaluation purpose and economic optimization for method development.
2. What is the basic idea behind the simulation used by PyroDoQ?
   The foundation for simulation of LAL-endotoxin kinetic reaction is derived from a 4-parameter model. The parameters are assigned to represent various characteristics of the measurement and expanded to include additional variables, such as noise, outliers, saturation, etc. The algorithm was refined and verified against experimental data and was proven to be a very effective tool for DQ & validation. The model:
                         
3. What is the advantage of simulation?
   There are two main advantages in using simulation:
   - Simulation needs a computer with appropriate software only.
   - By using simulation the extra time and cost due to unecessary experiments will be reduced.
4. Can I use PyroDoQ for DQ & validation of chromogenic and turbidimetric LAL-kinetic measurement techniques?
   Yes! The IQ database used to develop the simulation model was based on kinetic chromogenic measurements. Proportional adjustment of the parameters provided accurate modeling.
5. Is Signal Processing used in PyroDoQ & what is Signal Processing?
   Yes, Signal Processing option is used in PyroDoQ! Signal Processing (SP) is a set of complex mathematical operations on digital signals to improve the quality of the signals.
6. Can I simulate curve smoothing in PyroDoQ?
   Yes! Curve smoothing is a mathematical method of reducing the effect of noise. By utilizing curve smoothing in PyroDoQ you can simulate worst-case-scenario conditions relative to your test.
7. Can I simulate "noise" conditions in PyroDoQ?
   Yes, you can adjust the noise level based on real world experimental data stored in the database! Noise is the standard term used to describe random electronic signals detected by an instrument. The relationship between the useful signal and the noise is characterized by the signal-to-noise ratio (S/N). A low signal-to-noise ratio (where the noise component is high relative to the useful signal) may necessitate retesting. The unit used to describe the quality of a signal is the decibel (dB). Signals of 30 dB or below are too noisy to be useful.
8. Can I generate outlier(s) in PyroDoQ?
   Yes, you can introduce outliers based on real world experimental data stored in the database! The outlier is a value in a data set with very low statistical probability of belonging to the same population.
9. Can I simulate Robust Kinetic measurement?
   Yes, you can. The query is a sentence in natural language optionally containing parameters. These parameters are the parts of a sentence that can be modified by the user to obtain different results. When a query is executed, data from the database is selected and the results obtained are displayed in different windows (panels), such as chart, graph or sheet. The PyroDoQ contains this kind of query.
10. Can I use PyroDoQ for other applications?
    Yes. The simulation algorithms include models for detector suitability evaluation, noise effects, least quantifiable amounts, etc., that can be used generically.
11. Can I integrate PyroDoQ with other Opulus applications?
    Yes. Other Opulus applications, such as Epsilon for smart validation and others to come, can synergestically work together for expanded capabilities of DQ relative to systems other than LAL-endotoxin testing kinetics.